Search for: All records

Semi‐arid grasslands on the Mongolian Plateau are expected to experience high inputs of anthropogenic reactive nitrogen in this century. It remains unclear, however, how soil organisms and nutrient cycling are directly affected by N enrichment (i.e., without mediation by plant input to soil) vs. indirectly affected via changes in plant‐related inputs to soils resulting from N enrichment. To test the direct and indirect effects of N enrichment on soil organisms (bacteria, fungi and nematodes) and their associated C and N mineralization, in 2010, we designated two subplots (with plants and without plants) in every plot of a six‐level N‐enrichment experiment established in 1999 in a semi‐arid grassland. In 2014, 4 years after subplots with and without plant were established, N enrichment had substantially altered the soil bacterial, fungal and nematode community structures due to declines in biomass or abundance whether plants had been removed or not. N enrichment also reduced the diversity of these groups (except for fungi) and the soil C mineralization rate and induced a hump‐shaped response of soil N mineralization. As expected, plant removal decreased the biomass or abundance of soil organisms and C and N mineralization rates due to declines in soil substrates or food resources. Analyses of plant‐removal‐induced changes (ratios of without‐ to with‐plant subplots) showed that micro‐organisms and C and N mineralization rates were not enhanced as N enrichment increased but that nematodes were enhanced as N enrichment increased, indicating that the effects of plant removal on soil organisms and mineralization depended on trophic level and nutrient status. Surprisingly, there was no statistical interaction between N enrichment and plant removal for most variables, indicating that plant‐related inputs did not qualitatively change the effects of N enrichment on soil organisms or mineralization. Structural equation modelling confirmed that changes in soil communities and mineralization rates were more affected by the direct effects of N enrichment (via soil acidification and increased N availability) than by plant‐related indirect effects. Our results provide insight into how future changes in N deposition and vegetation may modify below‐ground communities and processes in grassland ecosystems. 

Abstract Livestock grazing has been shown to alter the structure and functions of grassland ecosystems. It is well acknowledged that grazing pressure is one of the strongest drivers of ecosystem‐level effects of grazing, but few studies have assessed how grazing pressure impacts grassland biodiversity and ecosystem multifunctionality (EMF).Here, we assessed how different metrics of biodiversity (i.e., plants and soil microbes) andEMFresponded to seven different grazing treatments based on an 11‐year field experiment in semi‐arid Inner Mongolian steppe.We found that soil organic carbon, plant‐available nitrogen and plant functional diversity all decreased even at low grazing pressure, while above‐ground primary production and bacterial abundance decreased only at high levels of grazing pressure.Structural equation models revealed thatEMFwas driven by direct effects of grazing, rather than the effects of grazing on plant or microbial community composition. Grazing effects on plant functional diversity and soil microbial abundance did have moderate effects onEMF, while plant richness did not.Synthesis. Our results showed ecosystem functions differ in their sensitivity to grazing pressure, requiring a low grazing threshold to achieve multiple goals in the Eurasian steppe. Aplain language summaryis available for this article. 

Nitrogen deposition, along with habitat losses and climate change, has been identified as a primary threat to biodiversity worldwide (Butchart et al., 2010; MEA, 2005; Sala et al., 2000). The source of this stressor to natural systems is generally twofold: burning of fossil fuels and the use of fertilizers in modern intensive agriculture. Each of these human enterprises leads to the release of large amounts of biologically reactive nitrogen (henceforth contracted to "nitrogen") to the atmosphere, which is later deposited to ecosystems. Because nitrogen is a critical element to all living things (as a primary building block of proteins among other biological molecules), nitrogen availability often limits primary production and is tightly recycled in many natural ecosystems. This is especially true in temperate ecosystems, though it may also be true for some areas in the tropics that are not phosphorus-limited (Adams et al., 2004; Matson et al., 1999). Thus, the large increase in availability of this critical nutrient as a result of human activity has profound impacts on ecosystems and on biodiversity. Once nitrogen is deposited on terrestrial ecosystems, a cascade of effects can occur that often leads to overall declines in biodiversity (Bobbink et al., 2010; Galloway et al., 2003). For plants, nitrogen deposition can impact biodiversity generally through four processes: (1) stimulation of growth often of weedy species that outcompete local neighbors (termed "eutrophication"), (2) acidification of the soil and consequent imbalances in other key nutrients that favors acid tolerant species (termed "acidification"), (3) enhancement of secondary stressors such as from fire, drought, frost, or pests triggered by increased nitrogen availability (termed "secondary stressors"), and (4) direct damage to leaves (termed "direct toxicity") (Bobbink, 1998; Bobbink et al., 2010). For animals, much less is known, but reductions in plant biodiversity can lead to reductions in diversity of invertebrate and other animal species, loss of habitat heterogeneity and specialist habitats, increased pest populations and activity, and changes in soil microbial communities (McKinney and Lockwood, 1999; Throop and Lerdau, 2004; Treseder, 2004). Citation Clark, Christopher M.; Bai, Yongfei; Bowman, William D.; Cowles, Jane M.; Fenn, Mark E.; Gilliam, Frank S.; Phoenix, Gareth K.; Siddique, Ilyas; Stevens, Carly J.; Sverdrup, Harald U.; Throop, Heather L. 2013. Nitrogen deposition and terrestrial biodiversity. In: Levin S.A. (ed.) Encyclopedia of Biodiversity, second edition, Volume 5, Waltham, MA: Academic Press. pp. 519-536.